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	Provedor de dados ArchiMer (1) International Journal of Morphology (1) Autor Goux, Didier (1) Idrus,Ruszymah B. H (1) Ledauphin, Jerome (1) Muhammed,Aisha A (1) Orvain, Francis (1) Saim,Aminuddin B (1) Takahashi, Eri (1) Yunus,Mohd Heikal M (1) Mais... Palavra-chave Immunostaining (2) Biofilm (1) Cell lysis (1) Dowex (1) GCMS analysis (1) Human nasal respiratory epithelium (1) Immuno staining (1) Intertidal sediments (1) Mesenchymal stem cells marker (1) Nasal turbinate (1) Navicula jeffreyi (1) Protein (1) Tissue engineering (1) Transmission electron microscopy (1) Mais... Tipo do documento Journal article (1) Text (1) Ano 2015 (1) 2009 (1) País Chile (1) France (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Expression of Mesenchymal Stem Cell Phenotype in Human Nasal Respiratory Epithelial Cells International Journal of Morphology Muhammed,Aisha A; Yunus,Mohd Heikal M; Saim,Aminuddin B; Idrus,Ruszymah B. H. The respiratory epithelium is the first line of contact with the external hazards. Thus it can be damaged and need to be replaced to avoid healing by fibrosis. Tracheal tissue engineering is an alternative promising treatment modality. Mesenchymal stem cell markers are surface proteins, which are responsible for some of these cells unique properties. The objective of this study was to detect the mesenchymal stem cell phenotype among the human nasal respiratory epithelial cells via two immunophenotyping techniques. Respiratory epithelial cells were cultured using co-culture technique, fibroblasts was removed at confluence leaving respiratory epithelial cells, which were passage further to passage 4. Cells were evaluated for mesenchymal stem cell markers... Tipo: Journal article Palavras-chave: Human nasal respiratory epithelium; Immunostaining; Mesenchymal stem cells marker; Tissue engineering; Nasal turbinate. Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000400047 Optimising extraction of extracellular polymeric substances (EPS) from benthic diatoms: comparison of the efficiency of six EPS extraction methods ArchiMer Takahashi, Eri; Ledauphin, Jerome; Goux, Didier; Orvain, Francis. There is no universal method that can be applied to extract bound extracellular polymeric substances (EPS) from benthic diatoms of intertidal sediments without causing cell lysis. Six extraction methods were tested on a diatom culture of Navicula jeffreyi to establish the best compromise between high yields of carbohydrate extraction and minimum cell lysis. Extraction with distilled water provoked cell lysis (as already known). The five other extraction methods (dowex resin, artificial seawater of half salinity and extractions after pretreatment with gluteraldehyde by three methods: water, dowex water and dowex buffer) did not provoke cell lysis as shown by transmission electronic microscopy. This result was confirmed by the minimum release of internal... Tipo: Text Palavras-chave: Transmission electron microscopy; Protein; Navicula jeffreyi; Intertidal sediments; Immuno staining; Immunostaining; GCMS analysis; Dowex; Cell lysis; Biofilm. Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-7418.pdf Registros recuperados: 2 Primeira ... 1 ... Última

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